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dc.contributor.authorLopez Senra, Estefanía 
dc.contributor.authorCasal Beiroa, Paula
dc.contributor.authorLópez Álvarez, Miriam 
dc.contributor.authorSerra Rodríguez, Julia Asunción 
dc.contributor.authorGonzález Fernández, Pío Manuel 
dc.contributor.authorValcarcel, Jesús
dc.contributor.authorVázquez, José Antonio
dc.contributor.authorBurguera, Elena F.
dc.contributor.authorBlanco, Francisco J.
dc.contributor.authorMagalhães, Joana
dc.date.accessioned2021-04-25T08:17:12Z
dc.date.available2021-04-25T08:17:12Z
dc.date.issued2020-01-31
dc.identifier.citationMarine Drugs, 18(2): 94 (2020)spa
dc.identifier.issn16603397
dc.identifier.urihttp://hdl.handle.net/11093/2021
dc.description.abstractOsteoarthritis is the most prevalent rheumatic disease. During disease progression, differences have been described in the prevalence of chondroitin sulfate (CS) isomers. Marine derived-CS present a higher proportion of the 6S isomer, offering therapeutic potential. Accordingly, we evaluated the effect of exogenous supplementation of CS, derived from the small spotted catshark (Scyliorhinus canicula), blue shark (Prionace glauca), thornback skate (Raja clavata) and bovine CS (reference), on the proliferation of osteochondral cell lines (MG-63 and T/C-28a2) and the chondrogenic differentiation of mesenchymal stromal cells (MSCs). MG-G3 proliferation was comparable between R. clavata (CS-6 intermediate ratio) and bovine CS (CS-4 enrichment), for concentrations below 0.5 mg/mL, defined as a toxicity threshold. T/C-28a2 proliferation was significantly improved by intermediate ratios of CS-6 and -4 isomers (S. canicula and R. clavata). A dose-dependent response was observed for S. canicula (200 µg/mL vs 50 and 10 µg/mL) and bovine CS (200 and 100 µg/mL vs 10 µg/mL). CS sulfation patterns discretely affected MSCs chondrogenesis; even though S. canicula and R. clavata CS up-regulated chondrogenic markers expression (aggrecan and collagen type II) these were not statistically significant. We demonstrate that intermediate values of CS-4 and -6 isomers improve cell proliferation and offer potential for chondrogenic promotion, although more studies are needed to elucidate its mechanism of action.spa
dc.description.sponsorshipXunta de Galicia | Ref. AGRUP 2015/05 (CICA-INIBIC)spa
dc.description.sponsorshipXunta de Galicia | Ref. Grant IN607A 2017/11spa
dc.description.sponsorshipXunta de Galicia | Ref. Grupos de Potencial Crecimiento IN607B 2018/19spa
dc.description.sponsorshipEuropean Commission | Ref. 0245_IBEROS_1_Espa
dc.language.isoengspa
dc.publisherMarine Drugsspa
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleImpact of prevalence ratios of chondroitin sulfate (CS)- 4 and -6 isomers derived from marine sources in cell proliferation and chondrogenic differentiation processesspa
dc.typearticlespa
dc.rights.accessRightsopenAccessspa
dc.identifier.doi10.3390/md18020094
dc.identifier.editorhttps://www.mdpi.com/1660-3397/18/2/94spa
dc.publisher.departamentoFísica aplicadaspa
dc.publisher.grupoinvestigacionNovos Materiaisspa
dc.subject.unesco22 Físicaspa
dc.subject.unesco2407.01 Cultivo Celularspa
dc.subject.unesco3312 Tecnología de Materialesspa
dc.date.updated2021-04-22T18:07:55Z
dc.computerCitationpub_title=Marine Drugs|volume=18|journal_number=2|start_pag=94|end_pag=spa
dc.referencesThis research was financially supported by INTERREG V-A POCTEP Program through European FEDER funds (0245_IBEROS_1_E), AE CICA-INIBIC (AGRUP 2015/05) through Xunta de Galicia funds, Grant IN607A 2017/11 from Axencia Galega de Innovación (GAIN) and Xunta de Galicia (Grupos de Potencial Crecimiento IN607B 2018/19). The Biomedical Research Network Center (CIBER) is an initiative from Instituto de Salud Carlos III (ISCIII).spa


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    Attribution 4.0 International (CC BY 4.0)
    Except where otherwise noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)