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dc.contributor.authorRodrigues Amorim, Daniela
dc.contributor.authorIglesias Martínez Almeida, Marta
dc.contributor.authorRivera Baltanás, Tania
dc.contributor.authorFernández Palleiro, Patricia
dc.contributor.authorFreiría Martínez, Luis
dc.contributor.authorRodríguez Jamardo, Cynthia
dc.contributor.authorComís Tuche, María
dc.contributor.authorVallejo Curto, María del Carmen
dc.contributor.authorÁlvarez Ariza, María
dc.contributor.authorLópez García, Marta
dc.contributor.authorHeras, Elena de las
dc.contributor.authorGarcía Caballero, Alejandro 
dc.contributor.authorOlivares, Jose Manuel
dc.contributor.authorSpuch Calvar, Carlos 
dc.date.accessioned2021-09-17T07:42:18Z
dc.date.available2021-09-17T07:42:18Z
dc.date.issued2021-08-07
dc.identifier.citationInternational Journal of Molecular Sciences, 22(16): 8499 (2021)spa
dc.identifier.issn14220067
dc.identifier.urihttp://hdl.handle.net/11093/2462
dc.description.abstractThe neurobiology of schizophrenia is multifactorial, comprising the dysregulation of several biochemical pathways and molecules. This research proposes a peripheral biomarker for schizophrenia that involves the second extracellular loop of norepinephrine transporter (NEText), the tropomyosin receptor kinase C (TrkC), and the neurotrophin-3 (NT-3) in T cells. The study of NEText, NT-3, and TrkC was performed in T cells and plasma extracted from peripheral blood of 54 patients with schizophrenia and 54 healthy controls. Levels of NT-3, TrkC, and NET were significantly lower in plasma and T cells of patients compared to healthy controls. Co-immunoprecipitation (co-IPs) showed protein interactions with Co-IP NEText–NT-3 and Co-IP NEText–TrkC. Computational modelling of protein–peptide docking by CABS-dock provided a medium–high accuracy model for NT-3–NEText (4.6935 Å) and TrkC–NEText (2.1365 Å). In summary, immunocomplexes reached statistical relevance in the T cells of the control group contrary to the results obtained with schizophrenia. The reduced expression of NT-3, TrkC, and NET, and the lack of molecular complexes in T cells of patients with schizophrenia may lead to a peripheral dysregulation of intracellular signaling pathways and an abnormal reuptake of norepinephrine (NE) by NET. This peripheral molecular biomarker underlying schizophrenia reinforces the role of neurotrophins, and noradrenergic and immune systems in the pathophysiology of schizophrenia.spa
dc.description.sponsorshipFundação para a Ciência e a Tecnologia | Ref. SFRH/BD/135623/2018spa
dc.description.sponsorshipInstituto de Salud Carlos III | Ref. P16/00405spa
dc.description.sponsorshipInstituto de Salud Carlos III | Ref. PI20/00937spa
dc.description.sponsorshipAxencia Galega de Innovación | Ref. IN607B 2018/17spa
dc.language.isoengen
dc.publisherInternational Journal of Molecular Sciencesspa
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleThe role of the second extracellular loop of norepinephrine transporter, neurotrophin-3 and tropomyosin receptor kinase C in T cells: a peripheral biomarker in the etiology of schizophreniaspa
dc.typearticlespa
dc.rights.accessRightsopenAccessspa
dc.identifier.doi10.3390/ijms22168499
dc.identifier.editorhttps://www.mdpi.com/1422-0067/22/16/8499spa
dc.publisher.departamentoComunicación audiovisual e publicidadespa
dc.publisher.departamentoDpto. Externospa
dc.publisher.grupoinvestigacionTEAM NANO TECH (Grupo de Nanotecnoloxía)spa
dc.subject.unesco32 Ciencias Médicasspa
dc.subject.unesco3207.11 Neuropatologíaspa
dc.subject.unesco3211 Psiquiatríaspa
dc.date.updated2021-09-17T07:34:48Z
dc.referencesThis research was funded by FCT, Fundação para a Ciência e Tecnologia, grant number SFRH/BD/135623/2018, ISCIII, Instituto de Salud Carlos III grants number P16/00405 and PI20/00937 and GAIN grant number IN607B 2018/17 and IN607B2021/12.spa


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    Attribution 4.0 International
    Except where otherwise noted, this item's license is described as Attribution 4.0 International