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dc.contributor.authorCalo Rodríguez, Jessica 
dc.contributor.authorBlanco Imperiali, Ayelén Melisa 
dc.contributor.authorComesaña Fernández, Sara 
dc.contributor.authorConde Sieira, Marta 
dc.contributor.authorMorais, Sofía
dc.contributor.authorSoengas Fernández, José Luis 
dc.date.accessioned2021-11-16T13:03:29Z
dc.date.available2021-11-16T13:03:29Z
dc.date.issued2021-03-02
dc.identifier.citationScientific Reports, 11(1): 4933 (2021)spa
dc.identifier.issn20452322
dc.identifier.urihttp://hdl.handle.net/11093/2687
dc.description.abstractThis study aimed to characterize amino acid sensing systems in the gastrointestinal tract (GIT) of the carnivorous fish model species rainbow trout. We observed that the trout GIT expresses mRNAs encoding some amino acid receptors described in mammals [calcium-sensing receptor (CaSR), G protein-coupled receptor family C group 6 member A (GPRC6A), and taste receptors type 1 members 1 and 2 (T1r1, T1r2)], while others [taste receptor type 1 member 3 (T1r3) and metabotropic glutamate receptors 1 and 4 (mGlur1, mGlur4)] could not be found. Then, we characterized the response of such receptors, as well as that of intracellular signaling mechanisms, to the intragastric administration of L-leucine, L-valine, L-proline or L-glutamate. Results demonstrated that casr, gprc6a, tas1r1 and tas1r2 mRNAs are modulated by amino acids in the stomach and proximal intestine, with important differences with respect to mammals. Likewise, gut amino acid receptors triggered signaling pathways likely mediated, at least partly, by phospholipase C β3 and β4. Finally, the luminal presence of amino acids led to important changes in ghrelin, cholecystokinin, peptide YY and proglucagon mRNAs and/or protein levels. Present results offer the first set of evidence in favor of the existence of amino acid sensing mechanisms within the fish GITeng
dc.language.isoengspa
dc.publisherScientific Reportsspa
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleFirst evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tracteng
dc.typearticlespa
dc.rights.accessRightsopenAccessspa
dc.identifier.doi10.1038/s41598-021-84303-9
dc.identifier.editorhttp://www.nature.com/articles/s41598-021-84303-9spa
dc.publisher.departamentoBioloxía funcional e ciencias da saúdespa
dc.publisher.grupoinvestigacionFisioloxía de Peixesspa
dc.subject.unesco2401.13 Fisiología Animalspa
dc.subject.unesco3105.07 Hábitos de Alimentaciónspa
dc.subject.unesco3105 Peces y Fauna Silvestrespa
dc.date.updated2021-11-16T10:47:53Z
dc.computerCitationpub_title=Scientific Reports|volume=11|journal_number=1|start_pag=4933|end_pag=spa
dc.referencesAuthors thank Dr Rita Angotzi from Instituto de Acuicultura Torre de la Sal-CISC (Spain) for providing phylogenetical information of T1R genes in fish.spa


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    Except where otherwise noted, this item's license is described as Attribution 4.0 International