An environmental ecocorona influences the formation and evolution of the biological corona on the surface of single-walled carbon nanotubes
UNIVERSAL IDENTIFIER: http://hdl.handle.net/11093/2740
EDITED VERSION: https://linkinghub.elsevier.com/retrieve/pii/S2452074821000240
UNESCO SUBJECT: 3105 Peces y Fauna Silvestre ; 2415 Biología Molecular ; 2401 Biología Animal (Zoología)
DOCUMENT TYPE: article
Nanomaterials (NMs) taken up from the environment carry a complex ecocorona consisting of dissolved organic matter. An ecocorona is assumed to influence the interactions between NMs and endogenous biomolecules and consequently affects the formation of a biological corona (biocorona) and the biological fate of the NMs. This study shows that biomolecules in fish plasma attach immediately (within <5 min) to the surface of SWCNTs and the evolution of the biocorona is a size dependent phenomenon. Quantitative proteomics data revealed that the nanotube size also influences the plasma protein composition on the surface of SWCNTs. The presence of a pre-attached ecocorona on the surface of SWCNTs eliminated the influence of nanotube size on the formation and evolution of the biocorona. Over time, endogenous biomolecules from the plasma partially replaced the pre-attached ecocorona as measured using a fluorescently labelled ecocorona. The presence of an ecocorona offers a unique surface composition to each nanotube. This suggests that understanding the biological fate of NMs taken up from the environment by organisms to support the environmental risk assessment of NMs is a challenging task because each NM may have a unique surface composition in the body of an organism.
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