Feeding and food availability modulate brain-derived neurotrophic factor, an orexigen with metabolic roles in zebrafish
UNIVERSAL IDENTIFIER: http://hdl.handle.net/11093/3871
EDITED VERSION: http://www.nature.com/articles/s41598-020-67535-z
UNESCO SUBJECT: 2401.13 Fisiología Animal ; 3105.07 Hábitos de Alimentación ; 3105 Peces y Fauna Silvestre
DOCUMENT TYPE: article
Emerging fndings point to a role for brain-derived neurotrophic factor (BDNF) on feeding in mammals. However, its role on energy balance is unclear. Moreover, whether BDNF regulates energy homeostasis in non-mammals remain unknown. This research aimed to determine whether BDNF is a metabolic peptide in zebrafsh. Our results demonstrate that BDNF mRNAs and protein, as well as mRNAs encoding its receptors trkb2, p75ntra and p75ntrb, are detectable in the zebrafsh brain, foregut and liver. Intraperitoneal injection of BDNF increased food intake at 1, 2 and 6 h post-administration, and caused an upregulation of brain npy, agrp and orexin, foregut ghrelin, and hepatic leptin mRNAs, and a reduction in brain nucb2. Fasting for 7 days increased bdnf and p75ntrb mRNAs in the foregut, while decreased bdnf, trkb2, p75ntra and p75ntrb mRNAs in the brain and liver. Additionally, the expression of bdnf and its receptors increased preprandially, and decreased after a meal in the foregut and liver. Finally, we observed BDNF-induced changes in the expression and/ or activity of enzymes involved in glucose and lipid metabolism in the liver. Overall, present results indicate that BDNF is a novel regulator of appetite and metabolism in fsh, which is modulated by energy intake and food availability
Files in this item
- Blanco_AyelénMelisa_2020_Fee_a ...