RT Journal Article
T1 Cyclodextrin-elicited Bryophyllum suspension cultured cells: enhancement of the production of bioactive compounds
A1 García Pérez, Pascual
A1 Losada Barreiro, Sonia
A1 Gallego Veigas, Pedro Pablo
A1 Bravo Díaz, Carlos Daniel
K1 2307 Química Física
K1 2417 Biología Vegetal (Botánica)
AB The rates of production of secondary metabolites obtained by employing conventional plant breeding may be low for practical purposes. Thus, innovative approaches for increasing their rates of production are being developed. Here, we propose the use of elicited plant suspension cultured cells (PSCC) with cyclodextrins (CDs) as an alternative method for the production of bioactive compounds from Bryophyllum species. For this purpose, we analyzed the effects of methyl–β–cyclodextrin and 2–hydroxypropyl–β–cyclodextrin on cell culture growth and on the intra- and extracellular production of phenols and flavonoids. Results clearly show that CDs enhance the biosynthesis of polyphenols by PSCC favoring their accumulation outside the cells. CDs shift the homeostatic equilibrium by complexing extracellular phenolics, causing stress in cells that respond by increasing the production of intracellular phenolics. We also analyzed the radical scavenging activity of the culture medium extracts against 2,2–diphenyl–1–pycrilhydrazyl (DPPH) radical, which increased with respect to the control samples (no added CDs). Our results suggest that both the increase in the production of polyphenols and their radical scavenging activity are a consequence of their inclusion in the CD cavities. Overall, based on our findings, CDs can be employed as hosts for increasing the production of polyphenols from Bryophyllum species.
PB International Journal of Molecular Sciences
SN 14220067
YR 2019
FD 2019-10-18
LK http://hdl.handle.net/11093/2044
UL http://hdl.handle.net/11093/2044
LA eng
NO International Journal of Molecular Sciences, 20(20): 5180 (2019)
NO Xunta de Galicia | Ref. ED431D 2017/18
DS Investigo
RD 13-sep-2024