RT Journal Article
T1 T follicular helper and T follicular regulatory cells have different TCR specificity
A1 Maceiras, Ana Raquel
A1 Almeida, Silvia Cristina Paiva
A1 Mariotti Ferrandiz, Encarnita
A1 Chaara, Wahiba
A1 Jebbawi, Fadi
A1 Six, Adrien
A1 Hori, Shohei
A1 Klatzmann, David
A1 Faro Rivas, Jose Manuel
A1 Graca, Luis
K1 2412 Inmunología
K1 2412.04 Formación de Anticuerpos
K1 2412.99 Otras
AB Immunization leads to the formation of germinal centres (GCs) that contain both T follicular helper (Tfh) and T follicular regulatory (Tfr) cells. Whether T-cell receptor (TCR) specificity defines the differential functions of Tfh and Tfr cells is unclear. Here we show that  antigenspecific T cells after immunization are preferentially recruited to the GC to become Tfh cells, but not Tfr cells. Tfh cells, but not Tfr cells, also proliferate efficiently on restimulation with the same immunizing antigen in vitro. Ex vivo TCR repertoire analysis shows that immunization induces oligoclonal expansion of Tfh cells. By contrast, the Tfr pool has a TCR repertoire that more closely  resembles that of regulatory T (Treg) cells. Our data thus indicate that the GC Tfh and Tfr pools are generated from distinct TCR repertoires, with Tfh cells expressing antigen-responsive TCRs to promote  antibody responses, and Tfr cells expressing potentially autoreactive TCRs to suppress autoimmunity.
PB Nature Communications
SN 20411723
YR 2017
FD 2017-04-21
LK http://hdl.handle.net/11093/2084
UL http://hdl.handle.net/11093/2084
LA eng
NO Nature Communications, 8(1): 15067.1-15067.12 (2017)
NO Fundação para Ciência e Tecnologia | Ref. PTDC/SAU-IMU/120225/2010
DS Investigo
RD 14-sep-2024