RT Journal Article
T1 Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection
A1 Mateos Martín, Jesús
A1 Estévez Martínez, Olivia
A1 González Fernández, Maria Africa
A1 Anibarro, Luis
A1 Pallarés, Ángeles
A1 Reljic, Rajko
A1 Mussá, Tufária
A1 Gomes Maueia, Cremildo
A1 Nguilichane, Artur
A1 Gallardo, José M
A1 Medina Méndez, María Isabel
A1 Carrera Mouriño, Mónica
K1 32 Ciencias Médicas
K1 3205.08 Enfermedades Pulmonares
K1 2410.07 Genética Humana
K1 2412 Inmunología
AB Tuberculosis (TB) is the most lethal infection among infectious diseases. The specific aim of this study was to establish panels of serum protein biomarkers representative of active TB patients and their household contacts who were either infected (LTBI) or uninfected (EMI-TB Discovery Cohort, Pontevedra Region, Spain). A TMT (Tamdem mass tags) 10plex-based quantitative proteomics study was performed in quintuplicate containing a total of 15 individual serum samples per group. Peptides were analyzed in an LC-Orbitrap Elite platform, and raw data were processed using Proteome Discoverer 2.1. A total of 418 proteins were quantified. The specific protein signature of active TB patients was characterized by an accumulation of proteins related to complement activation, inflammation and modulation of immune response and also by a decrease of a small subset of proteins, including apolipoprotein A and serotransferrin, indicating the importance of lipid transport and iron assimilation in the progression of the disease. This signature was verified by the targeted measurement of selected candidates in a second cohort (EMI-TB Verification Cohort, Maputo Region, Mozambique) by ELISA and nephelometry techniques. These findings will aid our understanding of the complex metabolic processes associated with TB progression from LTBI to active disease.
PB Scientific Reports
SN 20452322
YR 2020
FD 2020-03-02
LK http://hdl.handle.net/11093/2104
UL http://hdl.handle.net/11093/2104
LA eng
NO Scientific Reports, 10, 3844 (2020)
DS Investigo
RD 09-sep-2024