RT Journal Article
T1 The molecular basis of different approaches for the study of cancer stem cells and the advantages and disadvantages of a three-dimensional culture
A1 Cianciosi, Danila
A1 Ansary, Johura
A1 Forbes Hernández, Tamara Yuliett
A1 Regolo, Lucia
A1 Quinzi, Denise
A1 Gracia Villar, Santos
A1 Garcia Villena, Eduardo
A1 Tutusaus Pifarre, Kilian
A1 Álvarez Suárez, José Miguel
A1 Battino, Maurizio
A1 Giampieri, Francesca
K1 2407.01 Cultivo Celular
K1 3201.01 Oncología
K1 3207.03 Carcinogénesis
AB Cancer stem cells (CSCs) are a rare tumor subpopulation with high differentiation, proliferative and tumorigenic potential compared to the remaining tumor population. CSCs were first discovered by Bonnet and Dick in 1997 in acute myeloid leukemia. The identification and isolation of these cells in this pioneering study were carried out through the flow cytometry, exploiting the presence of specific cell surface molecular markers (CD34+/CD38−). In the following years, different strategies and projects have been developed for the study of CSCs, which are basically divided into surface markers assays and functional assays; some of these techniques also allow working with a cellular model that better mimics the tumor architecture. The purpose of this mini review is to summarize and briefly describe all the current methods used for the identification, isolation and enrichment of CSCs, describing, where possible, the molecular basis, the advantages and disadvantages of each technique with a particular focus on those that offer a three-dimensional culture.
PB Molecules
SN 14203049
YR 2021
FD 2021-04-29
LK http://hdl.handle.net/11093/2140
UL http://hdl.handle.net/11093/2140
LA eng
NO Molecules, 26(9): 2615 (2021)
DS Investigo
RD 14-may-2025