RT Journal Article
T1 Ultrasound-assisted water extraction of mastocarpus stellatus carrageenan with adequate mechanical and antiproliferative properties
A1 Torres Pérez, María Dolores
A1 Flórez Fernández, Noelia
A1 Domínguez González, Herminia
K1 3309.03 Antioxidantes en Los Alimentos
K1 3328.25 Extracción Sólido - Líquido
K1 3303.03 Procesos Químicos
AB Ultrasound-assisted water extraction was optimized to recover gelling biopolymers and antioxidant compounds from Mastocarpus stellatus. A set of experiments following a Box–Behnken design was proposed to study the influence of extraction time, solid liquid ratio, and ultrasound amplitude on the yield, sulfate content, and thermo-rheological properties (viscoelasticity and gelling temperature) of the carrageenan fraction, as well as the composition (protein and phenolic content) and antiradical capacity of the soluble extracts. Operating at 80 °C and 80 kHz, the models predicted a compromise optimum extraction conditions at ~35 min, solid liquid ratio of ~2 g/100 g, and ultrasound amplitude of ~79%. Under these conditions, 40.3% carrageenan yield was attained and this product presented 46% sulfate and good mechanical properties, a viscoelastic modulus of 741.4 Pa, with the lowest gelling temperatures of 39.4 °C. The carrageenans also exhibited promising antiproliferative properties on selected human cancer cellular lines, A-549, A-2780, HeLa 229, and HT-29 with EC50 under 51.9 μg/mL. The dried soluble extract contained 20.4 mg protein/g, 11.3 mg gallic acid eq/g, and the antiradical potency was equivalent to 59 mg Trolox/g.
PB Marine Drugs
SN 16603397
YR 2021
FD 2021-05-19
LK http://hdl.handle.net/11093/2185
UL http://hdl.handle.net/11093/2185
LA eng
NO Marine Drugs, 19(5): 280 (2021)
NO Ministerio de Ciencia e Innovación (España) | Ref. RTI2018-096376-B-I00
DS Investigo
RD 03-oct-2024