RT Journal Article
T1 Optimal recovery of valuable biomaterials, chondroitin sulfate and bioapatites, from central skeleton wastes of blue shark
A1 Vázquez, José Antonio
A1 Fraguas, Javier
A1 González Fernández, Pío Manuel
A1 Serra Rodríguez, Julia Asunción
A1 Valcarcel, Jesús
K1 3314 Tecnología Médica
K1 3312 Tecnología de Materiales
K1 3303 Ingeniería y Tecnología Químicas
AB The industrial filleting of blue shark (Prionace glauca) led to the generation of a large number of central skeletons of low interest to fishmeal plants handling such wastes. In this context, the present study describes the optimization of the hydrolysis process (pH 8.35, T 58 °C, 1% (v/w) of alcalase and t = 4 h) to produce chondroitin sulfate (CS) together with the recovery of bioapatites. Then, that hydrolysate was chemically treated with an optimal alkaline-hydroalcoholic-saline solution (0.48 M of NaOH, 1.07 volumes of EtOH and 2.5 g/L of NaCl) and finally purified by ultrafiltration-diafiltration (30 kDa) to obtain glycosaminoglycan with a purity of 97% and a productive yield of 2.8% (w/w of skeleton). The size of the biopolymer (CS) was of 58 kDa with prevalence of 6S-GalNAc sulfation (4S/6S ratio of 0.25), 12% of GlcA 2S-GalNAc 6S and 6% of non-sulfated disaccharides. Crude bioapatites were purified by pyrolysis and FT-Raman and XRD techniques confirm the presence of hydroxyapatite [Ca5(PO4)3(OH)], with a molar mass of 502.3 g/mol, embedded in the organic matrix of the skeleton. The mineralized tissues of blue shark are promising marine sources for the extraction of high value biomaterials with clinical application in bone and tissue regeneration and are still completely unexplored.
PB Polymers
SN 20734360
YR 2020
FD 2020-11-06
LK http://hdl.handle.net/11093/2238
UL http://hdl.handle.net/11093/2238
LA eng
NO Polymers, 12(11): 2613 (2020)
NO POCTEP 2015 | Ref. 0245_IBEROS_1_E
DS Investigo
RD 20-mar-2025