RT Journal Article
T1 Evaluation of matrix issues in the applicability of the neuro-2a cell based assay on the detection of CTX in fish samples
A1 Castro Alonso, David Miguel
A1 Manger, Ronald
A1 Vilariño Bermúdez, Óscar
A1 Gago Martínez, Ana María Consuelo
K1 3206.11 Toxicidad de Los Alimentos
K1 3105 Peces y Fauna Silvestre
K1 2301 Química Analítica
AB Ciguatoxins (CTXs) are a group of neurotoxins responsible for the syndrome ciguatera fish poisoning (CFP) as a result of the consumption of contaminated fish. The presence of these toxins has been detected around the Pacific, Caribbean and Indian coasts. Recent reports indicate the emergence of CFP in other geographic areas, in particular in European coasts, of the Canary Islands (Spain) and Madeira (Portugal). A neuroblastoma cell line of murine origin (N2a) has been applied to assay different groups of neurotoxins, acting on voltage-gated sodium channel (VGSC) of excitable cells, N2a-MTT. The great potential of N2a-MTT as a sensitive tool for the CTXs screening is clearly recognized, notably because it allows the detection of these toxins at levels below recommended as security levels. However, the complexity of the matrix is a critical point on the application of N2a-MTT, which needs to be evaluated. The aim of this work is to provide recommendations for an implemented N2a-MTT method for CTXs determination in fish that avoids matrix effects, particularly those related to high lipid content.
PB Toxins
SN 20726651
YR 2020
FD 2020-05-09
LK http://hdl.handle.net/11093/2590
UL http://hdl.handle.net/11093/2590
LA eng
NO Toxins, 12(5): 308 (2020)
NO European Food Safety Authority | Ref. GP/EFSA/AFSCO/2015/03
DS Investigo
RD 05-oct-2023