RT Journal Article
T1 Viral infection drives the regulation of feeding behavior related genes in salmo salar
A1 Muñoz, David
A1 Fuentes, Ricardo
A1 Carnicero, Beatriz
A1 Aguilar, Andrea
A1 Sanhueza, Nataly
A1 San Martín, Sergio
A1 Agurto, Cristian
A1 Donoso, Andrea
A1 Valdivia, Leonardo E.
A1 Míguez Miramontes, Jesús Manuel
A1 Tort, Lluis
A1 Boltana, Sebastián
K1 2401.13 Fisiología Animal
K1 3105 Peces y Fauna Silvestre
AB The feeding behavior in fish is a complex activity that relies on the ability of the brain to integrate multiple signals to produce appropriate responses in terms of food intake, energy expenditure, and metabolic activity. Upon stress cues including viral infection or mediators such as the proinflammatory cytokines, prostaglandins, and cortisol, both Pomc and Npy/Agrp neurons from the hypothalamus are stimulated, thus triggering a response that controls both energy storage and expenditure. However, how appetite modulators or neuro-immune cues link pathogenesis and energy homeostasis in fish remains poorly understood. Here, we provide the first evidence of a molecular linkage between inflammation and food intake in Salmon salar. We show that in vivo viral challenge with infectious pancreatic necrosis virus (IPNV) impacts food consumption by activating anorexic genes such as mc4r, crf, and pomcb and 5-HT in the brain of S. salar. At the molecular level, viral infection induces an overall reduction in lipid content in the liver, favoring the production of AA and EPA associated with the increment of elovl2 gene. In addition, infection upregulates leptin signaling and inhibits insulin signaling. These changes are accompanied by a robust inflammatory response represented by the increment of Il-1b, Il-6, Tnfa, and Pge2 as well as an increased cortisol level in vivo. Thus, we propose a model in which hypothalamic neurons respond to inflammatory cytokines and stress-related molecules and interact with appetite induction/inhibition. These findings provide evidence of crosstalk between pathogenesis-driven inflammation and hypothalamic–pituitary–adrenocortical axes in stress-induced food intake behavior in fish.
PB International Journal of Molecular Sciences
SN 14220067
YR 2021
FD 2021-10-21
LK http://hdl.handle.net/11093/2609
UL http://hdl.handle.net/11093/2609
LA eng
NO International Journal of Molecular Sciences, 22(21): 11391 (2021)
NO Comisión Nacional de Investigación Científica y Tecnológica, Chile | Ref. FONDECYT 1190627
DS Investigo
RD 01-oct-2023