RT Journal Article
T1 First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract
A1 Calo Rodríguez, Jessica
A1 Blanco Imperiali, Ayelén Melisa
A1 Comesaña Fernández, Sara
A1 Conde Sieira, Marta
A1 Morais, Sofía
A1 Soengas Fernández, José Luis
K1 2401.13 Fisiología Animal
K1 3105.07 Hábitos de Alimentación
K1 3105 Peces y Fauna Silvestre
AB This study aimed to characterize amino acid sensing systems in the gastrointestinal tract (GIT) of the carnivorous fish model species rainbow trout. We observed that the trout GIT expresses mRNAs encoding some amino acid receptors described in mammals [calcium-sensing receptor (CaSR), G protein-coupled receptor family C group 6 member A (GPRC6A), and taste receptors type 1 members 1 and 2 (T1r1, T1r2)], while others [taste receptor type 1 member 3 (T1r3) and metabotropic glutamate receptors 1 and 4 (mGlur1, mGlur4)] could not be found. Then, we characterized the response of such receptors, as well as that of intracellular signaling mechanisms, to the intragastric administration of L-leucine, L-valine, L-proline or L-glutamate. Results demonstrated that casr, gprc6a, tas1r1 and tas1r2 mRNAs are modulated by amino acids in the stomach and proximal intestine, with important differences with respect to mammals. Likewise, gut amino acid receptors triggered signaling pathways likely mediated, at least partly, by phospholipase C β3 and β4. Finally, the luminal presence of amino acids led to important changes in ghrelin, cholecystokinin, peptide YY and proglucagon mRNAs and/or protein levels. Present results offer the first set of evidence in favor of the existence of amino acid sensing mechanisms within the fish GIT
PB Scientific Reports
SN 20452322
YR 2021
FD 2021-03-02
LK http://hdl.handle.net/11093/2687
UL http://hdl.handle.net/11093/2687
LA eng
NO Scientific Reports, 11(1): 4933 (2021)
DS Investigo
RD 19-may-2025