RT Journal Article
T1 Strawberry tree honey in combination with 5-fluorouracil enhances chemosensitivity in human colon adenocarcinoma cells
A1 Afrin, Sadia
A1 Giampieri, Francesca
A1 Cianciosi, Danila
A1 Álvarez Suárez, José Miguel
A1 Bullón, Beatriz
A1 Amici, Adolfo
A1 Quiles, Josè L.
A1 Forbes Hernández, Tamara Yuliett
A1 Battino, Maurizio
K1 3207.13 Oncología
K1 2302.90 Bioquímica de Alimentos
K1 309.20 Propiedades de Los Alimentos
AB Colorectal cancer remains a challenging health burden worldwide. This study aimed to assess the potentiality of Strawberry tree honey (STH), a polyphenol-enriched food, to increase the effectiveness of 5-Fluorouracil (5-FU) in adenocarcinoma (HCT-116) and metastatic (LoVo) colon cancer cell lines. The combined treatment reduced cell viability and caused oxidative stress, by increasing oxidative biomarkers and decreasing antioxidant defence, in a more potent way compared to 5-FU alone. The expression of endoplasmic reticulum (ATF-6, XBP-1) and MAPK (p-p38 MAPK, p-ERK1/2) markers were also elevated after the combined treatment, enhancing the cell cycle arrest through the modulation of regulatory genes (i.e., cyclins and CDKs). Apoptotic gene (i.e., caspases) expressions were also increased after the combined treatment, while those of proliferation (i.e., EGFR), cell migration, invasion (i.e., matrix metallopeptidase) and epithelial–mesenchymal transition (N-cadherin, β-catenin) were suppressed. Finally, the combined treatment led cell metabolism towards a quiescent stage, by reducing mitochondrial respiration and glycolysis. In conclusion, this work represents an initial step to highlight the possibility to use STH in combination with 5-FU in the treatment of colon cancer, even if further in vitro an in vivo studies are strongly needed to confirm the possible chemo-sensitizing effects of STH.
PB Food and Chemical Toxicology: 02786915
SN 02786915
YR 2021
FD 2021-10
LK http://hdl.handle.net/11093/2753
UL http://hdl.handle.net/11093/2753
LA eng
NO Food and Chemical Toxicology, 156, 112484 (2021)
NO Financiado para publicación en acceso aberto: Universidade de Vigo/CISUG
DS Investigo
RD 08-sep-2024