RT Journal Article
T1 Understanding immune-modulatory efficacy in vitro
A1 Khatua, Somanjana
A1 Simal Gándara, Jesús
A1 Acharya, Krishnendu
K1 3209 Farmacología
K1 2412 Inmunología
AB Boosting or suppressing our immune system represents an attractive adjunct in the treatment of infections including SARS-CoV-2, cancer, AIDS, malnutrition, age related problems and some inflammatory disorders. Thus, there has been a growing interest in exploring and developing novel drugs, natural or synthetic, that can manipulate our defence mechanism. Many of such studies, reported till date, have been designed to explore effect of the therapeutic on function of macrophages, being a key component in innate immune system. Indeed, RAW264.7, J774A.1, THP-1 and U937 cell lines act as ideal model systems for preliminary investigation and selection of dose for in vivo studies. Several bioassays have been standardized so far where many techniques require high throughput instruments, cost effective reagents and technical assistance that may hinder many scholars to perform a method demanding compilation of available protocols. In this review, we have taken an attempt for the first time to congregate commonly used in vitro immune-modulating techniques explaining their principles. The study detected that among about 40 different assays and more than 150 sets of primers, the methods of cell proliferation by MTT, phagocytosis by neutral red, NO detection by Griess reaction and estimation of expression of TLRs, COX-2, iNOS, TNF-α, IL-6 and IL-1β by PCR have been the most widely used to screen the therapeutics under investigation.
PB Chemico-Biological Interactions
SN 00092797
YR 2022
FD 2022-01-25
LK http://hdl.handle.net/11093/3133
UL http://hdl.handle.net/11093/3133
LA eng
NO Chemico-Biological Interactions, 352, 109776 (2022)
NO Financiado para publicación en acceso aberto: Universidade de Vigo/CISUG
NO Universidade de Vigo/CISUG.
DS Investigo
RD 03-oct-2024