RT Journal Article
T1 Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates
A1 Terra Matos, Joana
A1 Teixeira, Marta Oliveira
A1 Santos Pereira, Cátia
A1 Noronha, Henrique
A1 Domingues, Lucília
A1 Sieiro Vázquez, María del Carmen
A1 Gerós, Hernâni
A1 Chaves, Susana Rodrigues
A1 Sousa, Maria João
A1 Côrte Real, Manuela
K1 2302 Bioquímica
K1 3302 Tecnología Bioquímica
AB Yeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Understanding the mechanisms involved in AA-induced RCD (AA-RCD) may thus help select robust fermentative yeast strains, providing novel insights to improve lignocellulosic ethanol (LE) production. Herein, we hypothesized that zinc vacuolar transporters are involved in vacuole-mediated AA-RCD, since zinc enhances ethanol production and zinc-dependent catalase and superoxide dismutase protect from AA-RCD. In this work, zinc limitation sensitized wild-type cells to AA-RCD, while zinc supplementation resulted in a small protective effect. Cells lacking the vacuolar zinc transporter Zrt3 were highly resistant to AA-RCD, exhibiting reduced vacuolar dysfunction. Moreover, zrt3Δ cells displayed higher ethanol productivity than their wild-type counterparts, both when cultivated in rich medium with AA (0.29 g L−1 h−1 versus 0.11 g L−1 h−1) and in an LH (0.73 g L−1 h−1 versus 0.55 g L−1 h−1). Overall, the deletion of ZRT3 emerges as a promising strategy to increase strain robustness in LE industrial production.
PB Journal of Fungi
SN 2309608X
YR 2022
FD 2022-01-14
LK http://hdl.handle.net/11093/3258
UL http://hdl.handle.net/11093/3258
LA eng
NO Journal of Fungi, 8(1): 78 (2022)
NO Fundação para a Ciência e Tecnologia | Ref. UIDB/04050/2020 - PE 20-23
DS Investigo
RD 26-sep-2023