RT Journal Article
T1 Protective effects of antioxidants on striatal dopamine release induced by organophosphorus pesticides
A1 Ferreira Faro, Lilian Rosana
A1 Costas Ferreira, María Del Carmen
A1 Pantoja, A.A.
A1 Duran Barbosa, Rafael
K1 3214 Toxicología
AB Although the toxic effects of organophosphorus (OP) pesticides have been classically attributed to inhibition of the acetylcholinesterase, other neurotoxic mechanisms, as oxidative stress can also occur. Here we evaluated if antioxidants prevent the excessive dopamine release induced by OP pesticides in conscious and freely moving rats, using cerebral microdialysis technique. Intrastriatal infusion of paraoxon (5 mM), glufosinate (10 mM) or glyphosate (5 mM) significantly increased the dopamine release (1006 ± 106%, 991 ± 142%, and 1164 ± 128%, relative to baseline, respectively). To evaluate if these increased dopamine release could be related to oxidative stress, we pretreated animals with antioxidants glutathione (GSH, 400 or 800 μM), dithiothreitol (DTT, 5 or 10 μM), trolox (1 or 3 mM), and α-lipoic acid (ALA, 400 or 800 μM) before administration of OP pesticides. Intrastriatal administration of the antioxidants GSH, DTT, trolox, and ALA was highly effective in preventing the glyphosate and glufosinate-induced dopamine overflow. However, only GSH (800 μM) significantly decreased the effect of paraoxon on dopamine levels. The high toxicity of this pesticide and the low concentrations used could explain this lack of effect in our experimental conditions. The fact that ROS scavengers prevent the excessive dopamine release induced by OP pesticides, further supports the view that dopamine overflow can cause neuronal damage mediated, at least in part, by oxidative stress.
PB Pesticide Biochemistry and Physiology
SN 00483575
YR 2022
FD 2022-03
LK http://hdl.handle.net/11093/3267
UL http://hdl.handle.net/11093/3267
LA eng
NO Pesticide Biochemistry and Physiology, 182, 105035 (2022)
NO Financiado para publicación en acceso aberto: Universidade de Vigo/CISUG
NO Xunta de Galicia | Ref. ED431B2019/33
DS Investigo
RD 15-sep-2024