RT Journal Article T1 Wastewater and marine bioindicators surveillance to anticipate COVID-19 prevalence and to explore SARS-CoV-2 diversity by next generation sequencing: one-year study A1 Novoa García, Beatriz A1 Ríos Castro, Raquel A1 Otero Muras, Irene A1 Alves Ferreira de Gouveia, Susana Margarida A1 Cabo , Adrián A1 Saco Beiroa, Amaro A1 Rey Campos, Magalí A1 Pájaro, Manuel A1 Fajar, Noelia A1 Aranguren, Raquel A1 Romero, Alejandro A1 Panebianco, Antonella A1 Valdés, Lorena A1 Payo, Pedro A1 Alonso, Antonio A. A1 Figueras Huerta, Antonio A1 Cameselle Fernández, Claudio K1 3308 Ingeniería y Tecnología del Medio Ambiente K1 3108.09 Virus K1 3202 Epidemiología AB This study presents the results of SARS-CoV-2 surveillance in sewage water of 11 municipalities and marine bioindicators in Galicia (NW of Spain) from May 2020 to May 2021. An integrated pipeline was developed including sampling, pre-treatment and biomarker quantification, RNA detection, SARS-CoV-2 sequencing, mechanistic mathematical modeling and forecasting. The viral load in the inlet stream to the wastewater treatment plants (WWTP) was used to detect new outbreaks of COVID-19, and the data of viral load in the wastewater in combination with data provided by the health system was used to predict the evolution of the pandemic in the municipalities under study within a time horizon of 7 days. Moreover, the study shows that the viral load was eliminated from the treated sewage water in the WWTP, mainly in the biological reactors and the disinfection system. As a result, we detected a minor impact of the virus in the marine environment through the analysis of seawater, marine sediments and, wild and aquacultured mussels in the final discharge point of the WWTP. PB The Science of The Total Environment SN 00489697 YR 2022 FD 2022-08-10 LK http://hdl.handle.net/11093/3441 UL http://hdl.handle.net/11093/3441 LA eng NO The Science of The Total Environment, 833, 155140 (2022) NO Financiado para publicación en acceso aberto: Universidade de Vigo/CISUG NO Xunta de Galicia | IN607B 2019/01 DS Investigo RD 06-oct-2024