RT Journal Article
T1 Pechiche (Vitex cymosa Berteo ex Speng), a nontraditional fruit from Ecuador, is a dietary source of phenolic acids and nutrient minerals, in addition to efficiently counteracting the oxidative-induced damage in human dermal fibroblasts
A1 Guevara, Mabel
A1 Valdés Silverio, Luis A
A1 Granda Albuja, María G
A1 Iturralde, Gabriel
A1 Jaramillo Vivanco, Tatiana
A1 Giampieri, Francesca
A1 Santos Buelga, Celestino
A1 González Paramás, Ana M
A1 Battino , Maurizio
A1 Álvarez Suárez, José Miguel
K1 3309.03 Antioxidantes en Los Alimentos
K1 3309.20 Propiedades de Los Alimentos
K1 3107.04 Fruticultura
AB Pechiche fruits (Vitex cymosa Berteo ex Speng) from Ecuador were studied to determine their phenolic acid profile, nutrient minerals and capacity to protect primary human dermal fibroblasts (HDFa) against oxidative-induced damage. Up to five phenolic acids were identified, with homovanillic acid as the main one. Vitamin C, β-carotene and lutein were also determined. Phosphorus and potassium were the main macrominerals, while iron was the principal micromineral. HDFa were preincubated with a crude pechiche extract (PCext) and then subjected to oxidative stress. The activity of five antioxidant enzymes, intracellular reactive oxygen species (ROS) and ATP levels and lipid peroxidation and protein oxidation were used as markers of oxidative damage. Preincubation with PCext for 24 h allowed for the significant reduction of intracellular ROS levels, improved the intracellular ATP levels and protected lipids and proteins against oxidative damage (p < 0.05). Additionally, preincubation with PCext was also able to significantly (p < 0.05) improve the activity of the antioxidant enzymes catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione transferase, compared to the stressed group without pretreatment. The results obtained in this study suggest the potential of pechiche as a source of bioactive compounds, as well as its beneficial effect against oxidative stress.
PB Antioxidants
SN 20763921
YR 2020
FD 2020-01-27
LK http://hdl.handle.net/11093/3574
UL http://hdl.handle.net/11093/3574
LA eng
NO Antioxidants, 9(2): 109 (2020)
NO MINECO | Ref. AGL2015-64522-C2
DS Investigo
RD 14-sep-2024