RT Journal Article
T1 Activation of TREK currents by riluzole in three subgroups of cultured mouse nodose ganglion neurons
A1 Fernández Fernández, Diego
A1 Cadaveira Mosquera, Alba
A1 Rueda Ruzafa, Lola
A1 Herrera Pérez, Salvador
A1 Veale, Emma L
A1 Reboreda Prieto, Antonio
A1 Mathie, Alistair
A1 Lamas Castro, José Antonio
K1 2411.12 Fisiología del Sistema Nervioso Central
K1 2490.01 Neurofisiología
AB Two-pore domain potassium channels (K2P) constitute major candidates for the regulation of background potassium currents in mammalian cells. Channels of the TREK subfamily are also well positioned to play an important role in sensory transduction due to their sensitivity to a large number of physiological and physical stimuli (pH, mechanical, temperature). Following our previous report describing the molecular expression of different K2P channels in the vagal sensory system, here we confirm that TREK channels are functionally expressed in neurons from the mouse nodose ganglion (mNG). Neurons were subdivided into three groups (A, Ah and C) based on their response to tetrodotoxin and capsaicin. Application of the TREK subfamily activator riluzole to isolated mNG neurons evoked a concentration-dependent outward current in the majority of cells from all the three subtypes studied. Riluzole increased membrane conductance and hyperpolarized the membrane potential by approximately 10 mV when applied to resting neurons. The resting potential was similar in all three groups, but C cells were clearly less excitable and showed smaller hyperpolarization-activated currents at -100 mV and smaller sustained currents at -30 mV. Our results indicate that the TREK subfamily of K2P channels might play an important role in the maintenance of the resting membrane potential in sensory neurons of the autonomic nervous system, suggesting its participation in the modulation of vagal reflexes.
PB PLoS ONE
SN 19326203
YR 2018
FD 2018-06-21
LK http://hdl.handle.net/11093/3709
UL http://hdl.handle.net/11093/3709
LA eng
NO PLoS ONE, 13(6): e0199282 (2018)
NO Ministerio de Economía y Competitividad | Ref. BFU2014-58999-P
DS Investigo
RD 05-oct-2024