RT Journal Article
T1 Constitutive expression of  SlMX1 gene improves fruit yield and quality, health-promoting compounds, fungal resistance and delays ripening in transgenic tomato plants
A1 Ewas, Mohamed
A1 Harlina, Putri Widyanti
A1 Shahzad, Raheel
A1 Khames, Eman
A1 Ali, Farhan
A1 Nishawy, Elsayed
A1 Elsafty, Nagwa
A1 Ibrahim, Hamdy M.
A1 Gallego Veigas, Pedro Pablo
K1 2417.19 Fisiología Vegetal
AB Tomato is one of the major economically domesticated crops, and it is extensively used in different ways and purposes worldwide. Cell metabolism is the central core of all the biological processes to sustain life including cell growth, differentiation, maintenance, and response to environmental stress. To evaluate how genetic engineering can improve tomato fruit metabolome, the transcriptomic and metabolomic datasets of two transgenic tomatoes (SlMX1 overexpression and RNAi lines) have been compared with wild-type. The combined results demonstrated that the constitutive expression of SlMX1 not only increased trichome formation, carotenoids, and terpenoids as has been stated in several studies, but has also up- and down-regulated the expression of multiple genes related to cell growth (cell wall turnover), primary (carbohydrates, vitamins, and phytohormones), and secondary (phenylpropanoids, carotenoids, and terpenoids) metabolism, cell signaling, and stress responses. These changes in gene expression due to the constitutive expression of SlMX1 promote the most important agroeconomic traits such as fruit yield and quality, biosynthesis of health-promoting phytochemicals (including phenolic acids, flavonoids, and anthocyanins), and finally, activate resistance to Botrytis cinerea and repress the expression of over-ripening-related genes, thus extending the fruit shelf-life. In conclusion, the traits improvement achieved by SlMX1 overexpression can be harnessed in molecular breeding programs to engineer fruit size and yield, induce health-promoting secondary metabolites, promote fungal resistance, and finally extend the fruit shelf-life.
PB Journal of Plant Interactions
SN 17429145
YR 2022
FD 2022-04-27
LK http://hdl.handle.net/11093/3947
UL http://hdl.handle.net/11093/3947
LA eng
NO Journal of Plant Interactions, 17(1): 517-536 (2022)
NO Xunta de Galicia | Ref. ED431E 2018/07
DS Investigo
RD 14-oct-2024