RT Journal Article
T1 Influence of enzymatic hydrolysis and molecular weight fractionation on the antioxidant and lipase / α-amylase inhibitory activities in vitro of watermelon seed protein hydrolysates
A1 Mirzapour Kouhdasht, Armin
A1 García Vaquero, Marco
A1 Eun, Jong Bang
A1 Simal Gándara, Jesús
K1 3309.03 Antioxidantes en Los Alimentos
K1 2302.02 Aminoácidos
K1 2302.24 Péptidos
AB This study aims to evaluate the potential in vitro antioxidant and anti-obesity activities of watermelon seed protein hydrolysates (WSPH) obtained using different combinations of enzymes alcalase–proteinase K (ALC-PK) and alcalase–actinidin (ALC-ACT). There was a direct relationship between the degree of hydrolysis (DH) and the biological activities of the WSPH, with the highest DPPH (approximately 85%) and lipase inhibitory activities (≈59%) appreciated at DH of 36–37% and 33–35% when using ALC-PK and ALC-ACT, respectively. Following molecular weight fractionation, the ALC-PK WSPH < 3 kDa (F1) assayed at 1 mg.mL−1 had the highest DPPH-radical scavenging (89.22%), ferrous chelating (FC) (79.83%), reducing power (RP) (A 0.51), lipase inhibitory (71.36%), and α-amylase inhibitory (62.08%) activities. The amino acid analysis of ALC-PK WSPH and its fractions revealed a relationship between the biological activity of the extracts and their composition. High contents of hydrophobic amino acids, arginine, and aromatic amino acids were related to high antioxidant, lipase inhibitory, and α-amylase inhibitory activities in the extracts, respectively. Overall, this study revealed that underutilized protein sources such as WSPH, using the appropriate combination of enzymes, could result in the generation of new ingredients and compounds with powerful antioxidant and anti-obesity activities with promising applications as nutraceuticals or functional foods.
PB Molecules
SN 14203049
YR 2022
FD 2022-11-15
LK http://hdl.handle.net/11093/4069
UL http://hdl.handle.net/11093/4069
LA eng
NO Molecules, 27(22): 7897 (2022)
NO Department of Agriculture Food and the Marine
DS Investigo
RD 15-sep-2024