RT Journal Article
T1 Handling, reproducing and cryopreserving five European sea Urchins (Echinodermata, Klein, 1778) for biodiversity conservation purposes
A1 Paredes Rosendo, Estefanía
A1 Campos Rosende, Sara
A1 Lago Dopico, Alba
A1 Bueno, Tracy
A1 Constensoux, Julien
A1 Costas, Damián
K1 2417.05 Biología Marina
K1 2401.19 Zoología Marina
K1 2407 Biología Celular
AB In this work, five local sea urchin species found in European waters were studied. Four were regular species: Sphaerechinus granularis, Psammechinus miliaris, Echinus esculentus (Linnaeus, 1758) and the edible sea urchin Paracentrotus lividus; and one was an irregular species, Echinocardium cordatum. These five species of sea urchins have been studied regarding their fertility, toxicity of cryoprotecting agents, cryopreservation of different cell types and chilling injury. The baseline fertility is similar in P. lividus, P. miliaris and S. granularis. Nonetheless, the sperm:egg ratio, contact time and development of the fertilization envelope would need to be studied further on a case-by-case basis. Sperm can be maintained inactively in the gonad (4 °C), and oocytes also maintain quality in sea water (4 °C), even after 72 h. Sperm was cryopreserved for four species with some post-thaw intra specific variability, and embryo cryopreservation was only possible for S. granularis. Overall, this study provided a wider vision of the biology and reproduction of these species that will help us develop tools for their biodiversity conservation through cryopreservation.
PB Animals
SN 20762615
YR 2022
FD 2022-11-16
LK http://hdl.handle.net/11093/4140
UL http://hdl.handle.net/11093/4140
LA eng
NO Animals, 12(22): 3161 (2022)
NO Financiaciado para publicación en acceso aberto: Universidade de Vigo/CISUG
NO Fundacion Biodiversidad
DS Investigo
RD 20-sep-2024