RT Journal Article
T1 Extraction of the wheat straw hemicellulose fraction assisted by commercial endo-xylanases. Role of the accessory enzyme activities
A1 Rodríguez Sanz, Andrea
A1 Fuciños Gonzalez, Clara
A1 Torrado Agrasar, Ana Maria
A1 Rua Rodriguez, María Luisa
K1 3302.99 Otras
AB Wheat straw is a highly promising raw material for bio-refinery strategies. Most of the literature related to lignocellulose fractionation focuses on cellulose purification and hemicellulose solubilization. Pre-treatments for hemicellulose solubilization without the formation of undesired products usually reach low extraction yields, which leaves an important hemicellulose fraction unused. In this work, we propose a mild process for the efficient extraction of the hemicellulose fraction of wheat straw assisted by partial enzymatic hydrolysis with three commercial endo-xylanase cocktails. A first step with alkali at 40 ºC helped to disrupt the lignocellulosic structure and removed 19% of lignin while maintaining most of the hemicellulose in the solid. The enzymatic step enabled reaching extraction yields of 59.8%, 51.9%, and 42.5% with Ultraflo L, Pentopan mono conc, and Shearzyme 500L, respectively. We also discuss the catalytic properties of each endo-xylanase, in particular, their adscription to the GH10 or GH11 glycosyl hydrolase family, and the relevant role of accessory enzymes.
PB Industrial Crops and Products
SN 09266690
YR 2022
FD 2022-05
LK http://hdl.handle.net/11093/4275
UL http://hdl.handle.net/11093/4275
LA eng
NO Industrial Crops and Products, 179, 114655 (2022)
NO Financiado para publicación en acceso aberto: Universidade de Vigo/CISUG
NO Agencia Estatal de Investigación | Ref. RTI2018–099249-B-I00
DS Investigo
RD 04-oct-2023