RT Journal Article
T1 Fatty acid sensing in the gastrointestinal tract of rainbow trout: different to mammalian model?
A1 Calo Rodríguez, Jessica
A1 Comesaña Fernández, Sara
A1 Alonso Gómez, Ángel
A1 Soengas Fernández, José Luis
A1 Blanco Imperiali, Ayelén Melisa
K1 2401.13 Fisiología Animal
K1 3105.07 Hábitos de Alimentación
K1 3105 Peces y Fauna Silvestre
AB It is well established in mammals that the gastrointestinal tract (GIT) senses the luminal presence of nutrients and responds to such information by releasing signaling molecules that ultimately regulate feeding. However, gut nutrient sensing mechanisms are poorly known in fish. This research characterized fatty acid (FA) sensing mechanisms in the GIT of a fish species with great interest in aquaculture: the rainbow trout (Oncorhynchus mykiss). Main results showed that: (i) the trout GIT has mRNAs encoding numerous key FA transporters characterized in mammals (FA transporter CD36 -FAT/CD36-, FA transport protein 4 -FATP4-, and monocarboxylate transporter isoform-1 -MCT-1-) and receptors (several free FA receptor -Ffar- isoforms, and G protein-coupled receptors 84 and 119 -Gpr84 and Gpr119-), and (ii) intragastrically-administered FAs differing in their length and degree of unsaturation (i.e., medium-chain (octanoate), long-chain (oleate), long-chain polyunsaturated (α-linolenate), and short-chain (butyrate) FAs) exert a differential modulation of the gastrointestinal abundance of mRNAs encoding the identified transporters and receptors and intracellular signaling elements, as well as gastrointestinal appetite-regulatory hormone mRNAs and proteins. Together, results from this study offer the first set of evidence supporting the existence of FA sensing mechanisms n the fish GIT. Additionally, we detected several differences in FA sensing mechanisms of rainbow trout vs. mammals, which may suggest evolutionary divergence between fish and mammals.
PB International Journal of Molecular Sciences
SN 14220067
YR 2023
FD 2023-02-21
LK http://hdl.handle.net/11093/4668
UL http://hdl.handle.net/11093/4668
LA eng
NO International Journal of Molecular Sciences, 24(5): 4275 (2023)
NO Xunta de Galicia | Ref. ED431B 2022/01
DS Investigo
RD 19-sep-2024