RT Journal Article
T1 Mechanistic insights of Cucumis melo L. seeds for gastrointestinal muscle spasms through calcium signaling pathway–related gene regulation networks in WGCNA and in vitro, in vivo studies
A1 Wahid, Muqeet
A1 Saqib, Fatima
A1 Akhtar, Saeed
A1 Ali, Anam
A1 Tallei, Trina Ekawati
A1 Simal Gándara, Jesús
K1 3309 Tecnología de los Alimentos
K1 3309.20 Propiedades de Los Alimentos
K1 3205.03 Gastroenterología
AB Background: In addition to the nutritional benefits of Cucumis melo L., herbalists in Pakistan and India employseeds to treat various ailments. This study aimed to determine the regulatory role of C. melo seeds in calciummediatedsmooth muscle contraction.Methods: We identified and quantified the phytochemicals of C. melo with LC ESI–MS/MS and HPLC, thenconducted in vitro and in vivo tests to confirm the involvement in smooth muscle relaxation. Then, diarrheapredominantirritable bowel syndrome gene datasets from NCBI GEO were acquired, DEGs and WGCNA followedby functional enrichment analysis. Next, molecular docking of key genes was performed.Results: The quantification of C. melo seeds revealed concentrations of rutin, kaempferol, and quercetin were702.38 μg/g, 686.29 μg/g, and 658.41 μg/g, respectively. In vitro experiments revealed that C. melo seeds had adose-dependent relaxant effect for potassium chloride (80 mM)–induced spastic contraction and exhibited calciumantagonistic response in calcium dose-response curves. In in vivo studies, Cm.EtOH exhibited antidiarrheal,antiperistaltic, and antisecretory effects. The functional enrichment of WGCNA and DEGs IBS-associated pathogenicgenes, including those involved in calcium-mediated signaling, MAPK cascade, and inflammatory responses.MAPK1 and PIK3CG were identified as key genes with greater binding affinity with rutin, quercitrin, andkaempferol in molecular docking.Conclusions: The bronchodilator and antidiarrheal effects of C. melo were produced by altering the regulatorygenes of calcium-mediated smooth contraction
PB Computers in Biology and Medicine
SN 00104825
YR 2023
FD 2023-03
LK http://hdl.handle.net/11093/4938
UL http://hdl.handle.net/11093/4938
LA eng
NO Computers in Biology and Medicine, 155, 106596 (2023)
NO Universidade de Vigo/CISUG
DS Investigo
RD 20-sep-2024