RT Journal Article
T1 An empirical test of the estimation of historical effective population size using
 Drosophila melanogaster
A1 Novo Gimenez, Irene
A1 Pérez Pereira, Noelia
A1 Santiago, Enrique
A1 Quesada Rodríguez, Humberto Carlos
A1 Caballero Rúa, Armando
K1 2409.03 Genética de Poblaciones
AB The availability of a large number of high-density markers (SNPs) allows the estimation of historical effective population size (Ne) from linkage disequilibrium between loci. A recent refinement of methods to estimate historical Ne from the recent past has been shown to be rather accurate with simulation data. The method has also been applied to real data for numerous species. However, the simulation data cannot encompass all the complexities of real genomes, and the performance of any estimation method with real data is always uncertain, as the true demography of the populations is not known. Here, we carried out an experimental design with Drosophila melanogaster to test the method with real data following a known demographic history. We used a population maintained in the laboratory with a constant census size of about 2800 individuals and subjected the population to a drastic decline to a size of 100 individuals. After a few generations, the population was expanded back to the previous size and after a few further generations again expanded to twice the initial size. Estimates of historical Ne were obtained with the software GONE both for autosomal and X chromosomes from samples of 17 individuals sequenced for the whole genome. Estimates of the historical effective size were able to infer the patterns of changes that occurred in the populations showing generally good performance of the method. We discuss the limitations of the method and the application of the software carried out so far
PB Molecular Ecology Resources
SN 1755098X
YR 2023
FD 2023-07-17
LK http://hdl.handle.net/11093/5039
UL http://hdl.handle.net/11093/5039
LA eng
NO Molecular Ecology Resources, 23(7): 1632-1640 (2023)
NO Ministerio de Ciencia, Innovación y Universidades | Ref. PID2020-114426GB- C21
DS Investigo
RD 13-sep-2024