RT Journal Article T1 Simple and rapid method for the simultaneous determination of cholesterol and retinol in meat using normal-phase HPLC technique A1 Domínguez Valencia, Rubén A1 Barba, Francisco J. A1 Centeno Domínguez, Juan Antonio A1 Putnik, Predrag A1 Alpas, Hami A1 Lorenzo Rodríguez, José Manuel K1 3309.20 Propiedades de Los Alimentos K1 3309.21 Alimentos Proteínicos K1 3104.10 Productos AB A direct saponification and normal-phase high-performance liquid chromatography (NP-HPLC) procedure was developed for simultaneous determination of cholesterol and retinol in meat. A normal-phase silica column fitted with diode array detection at 208 nm for cholesterol and fluorescence detection (λ-excitation 344 nm/λ-emission 472 nm) for retinol, with mobile phase consisting of 2% (v/v) 2-propanol in n-hexane was used. Cholesterol was eluted at 10 min and retinol at 12.66 min. High linearity (R2 > 0.9996 for both compounds) in calibration range was obtained. The LOD and LOQ values show the high sensitivity of the developed methodology for simultaneous determination of cholesterol and retinol in meat. Recovery results obtained in this study (98.67–102.14% for cholesterol and 91.72–98.27% for retinol) were between AOAC recommendations to validated method and were comparable to most recent studies in precision and accuracy. In addition, the present method showed high repeatability and reproducibility. As a general conclusion, the results indicate that the direct saponification, extraction, and HPLC analysis is an adequate method for cholesterol and retinol analysis in meat samples. PB Food Analytical Methods SN 19369751 YR 2018 FD 2018 LK http://hdl.handle.net/11093/5740 UL http://hdl.handle.net/11093/5740 LA eng NO Food Analytical Methods, 11(2): 319-326 (2018) NO Xunta de Galicia | Ref. IN607B 2016/28 DS Investigo RD 19-sep-2024