RT Journal Article T1 Modelling the biphasic growth and product formation by Enterococcus faecium CECT 410 in realkalized fed-batch fermentations in whey A1 Pérez Guerra, Nelson A1 Fajardo Bernárdez, Paula A1 Fuciños González, Clara A1 Rodríguez Amado, Isabel A1 Alonso González, Elisa A1 Torrado Agrasar, Ana María A1 Pastrana Castro, Lorenzo Miguel K1 3199 Otras Especialidades Agrarias K1 23 Química K1 2403 Bioquímica AB The influence of initial pH on growth and nutrient (total sugars, nitrogen, and phosphorous) consumption by Enterococcus faecium CECT 410 was studied during batch cultures in whey. With these data, two realkalized fed-batch fermentations were developed using different feeding substrates. The shift from homolactic to mixed acid fermentation, the biphasic kinetics observed for cell growth and nitrogen consumption and the increase in the concentrations of biomass and products (lactic acid, acetic acid, ethanol, and butane-2,3-diol) were the most noteworthy observations of these cultures. Modelling the fed-batch growth of Ent. faecium with the Logistic and bi-Logistic models was not satisfactory. However, biomass production was best mathematically described with the use of a double Monod model, which was expressed in terms of biomass, product accumulation, and nitrogen utilization. Product formation was successfully modelled with a modified form of the Luedeking and Piret model developed in this study. PB Journal of Biomedicine and Biotechnology SN 11107243 YR 2010 FD 2010 LK http://hdl.handle.net/11093/5874 UL http://hdl.handle.net/11093/5874 LA eng NO Journal of Biomedicine and Biotechnology, 2010(290286): 1-16 (2010) NO Instituto Nacional de Investigación y Tecnología Agraria | Ref. CAL01-045-C2-2 DS Investigo RD 13-oct-2024